genetic variability and bottleneck analysis in raeini goat population by microsatellite markers

Authors

بیژن محمودی

دانشجوی دکترای تخصصی، دانشگاه دولتی باکو مرتضی دلیری

استادیار، پژوهشگاه ملی مهندسی ژنتیک و زیست فن آوری رامین حبیبی

دانشجوی دکترای تخصصی، دانشگاه زابل

abstract

assessing genetic biodiversity and population structure of minor populations through the information provided by neutral molecular markers, allows determination of their extinction risk and to design strategies for their management and conservation. analysis of microsatellite loci is known to be highly informative in the reconstruction of the historical processes underlying the evolution and differentiation of animal populations. iranian goat populations are recognized as an invaluable component of the world’s goat genetic resources.  the aim of this work is to study the genetic status of raeini goat population through the analysis of molecular data from 13 microsatellites markers. the mean expected heterozygosity across loci within populations ranged from 0.716 to 0.852. genetic diversity measures revealed a good status of biodiversity in the raeini goat population. the observed number of alleles ranged from 4 (ilsts022) to 10 (bm121) with a total of 92 alleles and mean of 7.08 alleles across loci. the overall heterozygosity, pic and shannon index values were 0.808, 0.76 and 1.719 indicating high genetic diversity. only 5 out of 13 loci were in hardy-weinberg equilibrium. the mean fis was -0.016.  only 4 loci had positive fis values and 9 loci had negative values.  genetic bottleneck hypotheses were also explored. our data suggest that the raeini goats have not experienced a genetic bottleneck in the recent past.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Genetic variability and population structure of Raeini Cashmere goat breed assessed from pedigree analysis

Pedigree information which collected from 1979 to 2012 were used to study the genetic variability and population structure of Raeini Cashmere goat, taking kids born from 2009 to 2012 into account as reference population. Averages of inbreeding coefficients in whole and inbred populations were computed to be 0.04% and 4.41%, respectively. Considering the reference population, average inbreeding ...

full text

Genetic Characterization of Hamra Goat Population in Two Different Locations of Morocco Using Microsatellite Markers

In this study, genetic diversity of two different populations of Hamra goat breed of Morocco was investigated in 60 different samples (including 30 from Beni Arouss and 30 from Rommani) using fifteen microsatellite markers. A total of 145 alleles were detected with average number per locus of 8.67 and 8.07 in Beni Arouss and Rommani goats, respectively. The Shannon’s information index ranged fr...

full text

Genetic architecture and bottleneck analyses of Salem Black goat breed based on microsatellite markers

Aim: The present study was undertaken in Salem Black goat population for genetic analysis at molecular level to exploit the breed for planning sustainable improvement, conservation and utilization, which subsequently can improve the livelihood of its stakeholders. Materials and Methods: Genomic DNA was isolated from blood samples of 50 unrelated Salem Black goats with typical phenotypic feature...

full text

Genetic diversity and bottleneck analysis of Kilakarsal sheep by microsatellite markers

The genetic diversity in the Kilakarsal sheep population was analysed using 25 microsatellite markers by multiplex PCR in five panels and automated DNA sequencer. The number of alleles ranged from 3 to 13 with a mean of 7.6 across loci. The mean effective number of alleles was 3.88. The size and frequencies of the alleles ranged from 80 to 278 bp and from 0.0100 to 0.8000, respectively. The PIC...

full text

Genetic variability in Brazilian wheat cultivars assessed by microsatellite markers

Wheat (Triticum aestivum) is one of the most important food staples in the south of Brazil. Understanding genetic variability among the assortment of Brazilian wheat is important for breeding. The aim of this work was to molecularly characterize the thirty-six wheat cultivars recommended for various regions of Brazil, and to assess mutual genetic distances, through the use of microsatellite mar...

full text

Genetic analysis of pike-perch, Sander lucioperca L., populations revealed by microsatellite DNA markers in Iran

This study was conducted in order to investigate genetic diversity and population structure of pike-perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of microsatellite primers, Polymerase Chain Reaction (...

full text

My Resources

Save resource for easier access later


Journal title:
علوم دامی

جلد ۲۷، شماره ۱۰۳، صفحات ۶۱-۷۰

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023